Association of common IL-10 promoter gene variants with the susceptibility to head and neck cancer in Tunisia

Lamia Makni,Oussema Souaiai,Abrar Al Ansari,Besma Yacoubi-Loueslati,Wassim Y Almawi,Cherif Ben Hamda,Ezzedine Gazouani,Amel Mezlini

doi:10.3906/sag-1805-21

Abstract

Background/aimWe investigated the association of three IL-10 promoter single-nucleotide polymorphisms and altered IL-10 plasma levels with the risk of head and neck cancer (HNC). Materials and methodsStudy subjects comprised 194 HNC patients [137 nasopharyngeal cancer (NPC) and 57 laryngeal cancer (LC)], and 263 healthy controls. Genotyping of rs1800896 (-1082A>G), rs1800871 (-819C>T), and rs1800872 (-592A>C) IL-10 variants was performed by real-time PCR; IL-10 levels were measured by enzyme amplified immuno sensitivity assay (EAISA). Results Carriage of rs1800896 A/A genotype was more frequent in the HNC and NPC cases, but was less frequent in the controls than the LC patients. Significant differences in IL-10 levels were observed between the rs1800896A/G genotype-carrying NPC patients and the controls. Positive association with NPC and LC was observed for rs1800871C/C, and carriage of rs1800872A/A genotype, and A allele were associated with higher risk of HNC and NPC, but not LC. GT rs1800896-rs1800871 haplotype was more frequent among the HNC and NPC patients than the controls in contrast to GC haplotype, which has a protective effect. Positive association was found between TA haplotype and LC. ConclusionOur results demonstrate that IL-10-1082, IL-10-819, and IL-10-592 variants, and haplotypes GC and GT constitute biomarkers for early detection of HNC, especially NPC subtype. IL-10 -819T/C and TA haplotype may be used as biomarkers for early detection of LC.

Highlights

Recent evidence supports increased head and neck cancer (HNC) incidence worldwide, with estimated 400,000–600,000 new cases per year, and 223,000– 300,000 annual deaths [1,2]
Carriage of rs1800896 A/A genotype was more frequent in the HNC and nasopharyngeal carcinoma (NPC) cases, but was less frequent in the controls than the laryngeal cancer (LC) patients
Positive association with NPC and LC was observed for rs1800871C/C, and carriage of rs1800872A/A genotype, and A allele were associated with higher risk of HNC and NPC, but not LC

Summary

Introduction

Recent evidence supports increased head and neck cancer (HNC) incidence worldwide, with estimated 400,000–600,000 new cases per year, and 223,000– 300,000 annual deaths [1,2]. HNC arises from malignant transformation of upper respiratory tract epithelial cells [3], and nasopharyngeal carcinoma (NPC) and laryngeal cancer (LC) are the most prevalent forms of HNC [4]. NPC and LC are anatomically and histologically related, they differ in the pathogenesis, biology, treatment, mortality, and morbidity, as well as geographic and ethnic incidence [5,6,7]. HNCs are multifactorial malignancies, which are influenced by environmental, viral, immunogenetic, and lifestyle risk factors [8,9,10]. Few studies addressed the implication of these factors in the variability of and the relative susceptibility to NPC and LC within different populations. Interleukin-10 (IL10) was described as a key player in the pathogenesis and progression of NPC and related malignancies [13]

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