Association of cholesteryl ester transferyl protein gene (G277A) polymorphism and risk prediction of type 2 diabetes mellitus: A case control study of Northern Indian population

Abstract

BackgroundSeveral gene polymorphisms have been associated with type 2 diabetes mellitus (T2DM) in different ethnic groups. Cholesteryl ester transferyl proteins (CETP) promote efflux of excess cholesterol from peripheral tissues back to liver for excretion. Low (Paraoxonase 1) PON1 and (High density lipoprotein) HDL level might decrease its protection and results in T2DM. Based on this, we tried to investigate the Cholesteryl ester transferyl protein gene polymorphism in association with T2DM and lipid traits. MethodsIn the present case-control study, 300 type 2 diabetes mellitus patients and 300 healthy volunteer were recruited. The CETP (G277A) genotypes were determined by RFLP-PCR, human serum PON1 level was measured by ELISA kit and biochemical analysis was done using standard protocol. ResultsThe frequencies of B1B1, B1B2 and B2B2 genotypes 24%, 62%, 14% and 22%, 60%, 18% were observed in control and T2DM respectively, B2 allele was found higher in type 2 diabetes mellitus, but no significant association was detected. We found B2B2 genotype had significantly associated with higher HDL level in both and case compared to B1B1. We also observed high PON1 level in B2B2 genotype in both cases and control. ConclusionIn our study CETP (G277A) gene polymorphism is not associated with T2DM but significantly affect various lipid traits and PON1 levels.