Abstract
Objective. This in vitro study investigated the effect of Fluoride (F), Chlorhexidine (CHX), and their association on the viability of Streptococcus mutans using a biofilm model. Materials and Methods. Biofilms were anaerobically grown on glass slides that were vertically suspended in 24-well plates for 5 days. After 48 h of initial growth, biofilms were treated for the next 72 h, 2x/day with CHX at 0.12% and 2%, F as NaF at 0.08% and 0.4% and their association. Results. CHX treatment decreased the bacteria counts either alone or in association with both F concentrations, when compared with control group and the F treatments alone (p<0.05). Conclusion. No addition effect was observed when CHX and F were used in combination, when compared with CHX used alone.
Highlights
S . mutans are closely related with the development of dental caries due their acidogenic and aciduric properties and their capacity to use dietary carbohydrates to synthesize extracellular polysaccharides
In high concentrations, F alters the bacterial metabolism by a direct inhibitory effect on the pumping protons associated with membrane H + ATPase [5]
Viable bacteria counts of biofilm treated with 0.08% NaF did not differ from those of the control
Summary
S . mutans are closely related with the development of dental caries due their acidogenic and aciduric properties and their capacity to use dietary carbohydrates to synthesize extracellular polysaccharides. Mutans are closely related with the development of dental caries due their acidogenic and aciduric properties and their capacity to use dietary carbohydrates to synthesize extracellular polysaccharides. They are considered the most cariogenic microorganisms in dental biofilm [1]. Many agents have been developed to act on deremineralization process or interfere on the metabolism/growth of dental biofilms [3]. In high concentrations, F alters the bacterial metabolism by a direct inhibitory effect on the pumping protons associated with membrane H + ATPase [5]. Little is known about the effect of high-F dentifrice on metabolism of oral bacteria
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