Abstract
A phytoplasma was identified in white willow trees exhibiting symptoms of proliferation, growing in urban areas in Hamedan province of Iran. The association of a phytoplasma with this disease was confirmed by nested PCR of the 16S rRNA gene using universal phytoplasma-specific primer pair P1/P7 and R16mF2n/R2. Results of nested-PCR amplified a product of expected size (1.4 Kb). Comparison of the sequencing results with those available in GenBank and phylogenetic analysis allowed classification of the phytoplasma into ‘Candidatus phytoplasma trifolii’ (16SrVI) taxonomic group. To the best of our knowledge, this is the first report on the molecular detection and identification of a clover proliferation group phytoplasma in willow trees in Iran.
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