Association of Calpain-10 gene (rs2975760 and rs3792267) Polymorphism with Type 2 Diabetes Mellitus in the Iraqi Population

Abstract

Background: Type II Diabetes Mellitus (T2DM) can be considered as the main diabetes’ type, which ispresent in all populations worldwide and all regions. Calpain-10 is a part of a vast intracellular proteasefamily. CAPN10 gene polymorphisms have been related to complex types of T2DM.Objective: the major goal of the presented work is evaluating the relation regarding CAPN10 genepolymorphisms (SNP44 rs2975760 and SNP43 rs3792267) with T2DM in the Iraqi population as well aschanges in serum lipid concentration and insulin concentration.Materials and Methods: Two groups of persons were recruited, 300 patients with type2 diabetes mellitus,and 300 healthy control individuals. Fasting serum glucose and serum lipid concentrations have beenevaluated via standard enzymatic approaches, while the concentrations of serum insulin were evaluated viaELISA assay. Genotyping of rs2975760 and rs3792267 SNPs is conducted via PCR-RFLP.Results: Those of the SNP-44 showed that patients of heterozygous genotype (TC) decreased significantlywith respect to the control group. Patients with the homozygous genotype (CC) elevated insignificantlyrelative to the control group. The minor allele C frequency in patients (12%) is decreased considerablyin the patients’ group relative to the group of controls (15%). The genotype results of SNP-43 illustratedthat patients of heterozygous (GA) genotypes decreased significantly with respect to the control group.Patients of the homozygous genotype (AA) appeared to be insignificantly higher than the controls. Theminor allele A frequency in patients (11.5%) is decreased considerably in the patients’ group relative to thegroup of controls (27%). Serum lipid concentrations, insulin, and insulin resistance are distributed in groupsof various genotypes of the 2 SNPs deferentially.Conclusion: SNP-44 and SNP-43 in Iraqi individuals are protective against the development of T2DM.They were implicated in serum lipid changes, insulin and insulin resistance values.