Association of breast cancer with human papillomavirus (HPV) infection in Northeast Brazil: molecular evidence

José Roosevelt Cavalcante,Luiz Gonzaga Porto Pinheiro,Paulo Roberto Carvalho De Almeida,Márcia Valéria Pitombeira Ferreira,Gizele Almada Cruz,Thales Alves Campelo,Caroliny Soares Silva,Luana Nepomuceno Gondim Costa Lima,Bruno Masato Kitagawa De Oliveira,Lara Mulato Lima,Laura Magda Costa Feitosa,Agostinho Câmara Pinheiro,Cristiane Cunha Frota

doi:10.6061/clinics/2018/e465

José Roosevelt Cavalcante, Luiz Gonzaga Porto Pinheiro + Show 11 more

Open Access

https://doi.org/10.6061/clinics/2018/e465

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Journal: Clinics	Publication Date: Jan 1, 2018
Citations: 22	License type: cc-by

Affiliation: Universidade Federal do Ceará

Abstract

OBJECTIVE:The aim of this study is to investigate the presence of human papillomavirus DNA and genotypes in breast cancer and normal breast tissue samples obtained from women from the northeast region of Brazil.METHOD:One hundred three breast cancer samples and 95 normal breast samples, as the non-malignant controls, were studied. DNA extraction was verified by human beta-globin gene amplification, and polymerase chain reaction was conducted based on HPV L1-specific consensus primers MY09/MY11 and GP5+/GP6+, followed by nested multiplex polymerase chain reaction with type-specific primers for the E6/E7 consensus region.RESULTS:Human papillomavirus DNA was detected in 51 (49.5%) breast carcinoma samples and 15 (15.8%) normal breast samples (p<0.0001). Human papillomavirus genotypes 6 and 11 were identified in 15.2% of all samples.CONCLUSIONS:The high frequency of human papillomavirus infection in breast cancer samples indicates a potential role of this virus in breast carcinogenesis in the studied participants.

Highlights

Breast cancer (BC) is the most common tumour among women throughout the world in both developed and developing countries
In the MY/GP Polymerase chain reaction (PCR) assay, 43.7% (45/103) of the BC samples were positive for human papilloma viruses (HPVs) DNA, whereas 7.4% (7/95) of the normal breast (NB) samples were positive for HPV DNA
8.4% (8/95) of the NB samples were positive for HPV DNA in the E6/E7 assay

Summary

Introduction

Breast cancer (BC) is the most common tumour among women throughout the world in both developed and developing countries. 1.79 million new cases of BC were predicted for 2015, with an estimated death count of 560,407 for the same year [1]. In Brazil, approximately 57,960 new cases of BC were expected in 2016, which is equivalent to an estimated risk of 5,620 for each 100,000 women [2]. Some viruses may have a role in BC, including mouse mammary tumour virus (MMTV), bovine leukaemia virus (BLV), human papilloma viruses (HPVs), and Epstein-Barr virus (EBV) [4]. Regarding HPV and BC, it has been demonstrated that HPV types 16 and 18 can immortalize normal breast (NB) epithelium [5].

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