Association of anti-oxidative capacity of HDL with subclinical atherosclerosis in subjects with and without non-alcoholic fatty liver disease

Hossein Poustchi,Amir Reza Radmard,Sara Karami,Abbas Pakdel,Fatemeh Ali Yari,Negar Sarmadi,Parisa Shabani

doi:10.1186/s13098-021-00741-5

Abstract

BackgroundNon-alcoholic fatty liver disease (NAFLD) patients are at a substantial risk for developing cardiovascular disease (CVD). High-density lipoprotein (HDL) is well known to have protective effects against the development of atherosclerotic CVD. One of the major antiatherogenic effects of HDL is its anti-oxidative function.ObjectivesThis study investigated the association of anti-oxidative capacity of HDL with subclinical atherosclerosis in NAFLD and non-NAFLD subjects.MethodsA total of 143 subjects including 51 NAFLD and 92 control subjects were included in this case–control study. HDL oxidative index (HOI) was determined spectrophotometrically using a cell-free method in the presence of a fluorescent substrate dichlorofluorescein diacetate (DCFDA). Paraoxonase 1 (PON1) activity, superoxide dismutase (SOD) activity, and malondialdehyde (MDA) plasma levels were assessed in both groups.ResultsThe NAFLD patients with impaired HDL anti-oxidative function (HOI ≥ 1) had higher MDA levels, aspartate amino transferase (AST), liver stiffness (LS), and carotid intima-media thickness (cIMT) values compared to the controls. HDL oxidative index (HOI) was positively correlated with MDA levels and cIMT and negatively correlated with SOD activity.ConclusionsHigher circulating levels of MDA were associated with the impaired anti-oxidative function of HDL in NAFLD. The impaired anti-oxidative capacity of HDL might be related to NAFLD severity and subclinical atherosclerosis in NAFLD patients.

Highlights

Non-Alcoholic Fatty Liver Disease (NAFLD) is the most leading cause of chronic liver disease which affects an estimated 20–30% of the general population [1]
The Non-alcoholic fatty liver disease (NAFLD) patients with impaired High-density lipoprotein (HDL) anti-oxidative function (HOI ≥ 1) had higher MDA levels, aspartate amino transferase (AST), liver stiffness (LS), and carotid intima-media thickness values compared to the controls
Higher circulating levels of MDA were associated with the impaired anti-oxidative function of HDL in NAFLD

Summary

Introduction

Non-Alcoholic Fatty Liver Disease (NAFLD) is the most leading cause of chronic liver disease which affects an estimated 20–30% of the general population [1]. Despite liver-related morbidity and mortality, cardiovascular disease (CVD) is the most. NAFLD patients exhibit atherogenic dyslipidemia with low levels of high-density lipoprotein cholesterol (HDLC) [3]. Several studies demonstrated an association of higher plasma high-density lipoprotein (HDL) cholesterol levels with a lower risk of atherosclerotic cardiovascular disease (CVD) morbidity and mortality. Recent studies suggest that HDL particle functionality might be a better predictor of CDV risk than HDL cholesterol mass levels [4]. One of the important antiatherogenic properties of HDL is its anti-oxidative function, the ability to suppress LDL oxidation which in turn decreases the atherogenicity of LDL particles [5]. Non-alcoholic fatty liver disease (NAFLD) patients are at a substantial risk for developing cardiovascular disease (CVD). High-density lipoprotein (HDL) is well known to have protective effects against the development of atherosclerotic CVD. One of the major antiatherogenic effects of HDL is its anti-oxidative function

Objectives

Methods

Conclusion