Association of Angiotensinogen T174M and M235T Gene Variants with Development of Hypertension in Turkish Subjects of Trakya Region

Abstract

ABSTRACTGenetic determinations of human essential (primary) hypertension are discussed by reviewing the candidate genes. Angiotensinogen (AGT) gene, coding the precursor of potent vasoactive hormone angiotensin II, in renin-angiotensin-system (RAS) has been reported to be associated with the onset of hypertension. The aim of this study was to investigate the role of variation in the 174 and 235 sites in exon 2 in AGT gene in the developing of primary hypertension in Turkish subjects from Trakya region.Our study involved 136 subjects, 84 hypertensive and 52 gender and age matched controls. T174M and M235T polymorphisms of the AGT gene were investigated using allele specific polymerase chain reaction (PCR) assay and restriction fragment length polymorphism (RFLP).The frequency of genotypes of the variant T174M in the patients with primary hypertension was TT=%73.8, TM=%26.2, and MM=%0.0, that were not different from the controls TT=%73.1, TM=%25.0, and MM=%1.9. And for M235T; the genotype frequencies in patients with primary hypertension were MM=%19.0 MT=%54.8, and TT=%26.2, which were again not significantly different from that of the controls MM=%26.9 MT=%46.2 and TT=%26.9.In conclusion this study shows that T174M and M235T variants of the AGT gene were not associated with primary hypertension in Turkish subjects from Trakya region.