Association of AGER gene G82S polymorphism with the severity of coronary artery disease in Chinese Han population

Abstract

Advanced glycosylation end-product receptor (AGER) plays an important role in the development and progression of atherosclerosis. The G82S polymorphism (rs2070600) is located in the ligand-binding V-domain of AGER suggesting a possible influence of this variant on AGER function. The aim of this study is to evaluate the association of the G82S polymorphism with the severity of coronary artery disease (CAD) in patients with or without type 2 diabetes mellitus (T2DM). The AGER gene G82S polymorphism was analysed in 270 nondiabetic and 270 type 2 diabetic Chinese Han patients with angiographically proven CAD (luminal stenosis ≥ 50%). The number of diseased vessels and Gensini score were used to determine the severity of CAD. Genotyping for the G82S polymorphism was performed by PCR-RFLP using restriction enzymes AluI. The frequency of 82S allele was significantly lower in the diabetic CAD patients with multi-vessel disease than in those with single-vessel disease (P = 0·015). When controlled for confounding variables, the 82S allele was associated with decreased risk of multi-vessel disease [P = 0·038, adjusted OR = 0·565 (0·329-0·972)]. The protective effect of the 82S allele against the severity of CAD was not observed in patients with nondiabetic CAD. AGER 82S allele showed a protective effect on CAD severity in the presence of T2DM in Chinese Han population.