Abstract
Association of actin filaments and intermediate, 10 nm filaments with the dense bodies in smooth muscle cells of the chicken gizzard was studied by thin-section and freeze-etch-replica electron microscopy. For thin-section electron microscopy we used the isolated dense bodies with attached filaments. Actin filaments appeared to be inserted into both ends (poles) of individual oblong dense bodies in such a way that arrowheads with HMM S-1 pointed away from the dense body. 10 nm filaments were attached laterally to the dense body in a side-to-side fashion. This site-specific association of actin and 10 nm filaments with the dense body was confirmed by the freeze-etch replica observations on Triton-treated smooth muscles.
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