Association of A53T polymorphism with Parkinson' s disease in Chinese patients

Abstract

Objective To investigate the association of α-synaptophysin A53T gene polymorphism with parkinson's disease(PD) in Chinese people. Methods The conventional polymerase chain reaction was used to detect the α-synaptophysin A53T gene polymorphism in 224 sporadic PD patients(PD group)and 154 healthy individuals(control group). According to the Hoehn-Yahr(H-Y) classification standard, PD patients were divided into H-Y≥3 group(n=172) and H-Y≤2.5 group(n=52). Each genotype and alleles frequencies as well as the A53T gene expression and their relation to the severity of parkinson's disease were analyzed with Chi-square test of SPSS19.0. Results The frequency of the A53T genotype of A/A were 40(17.9%) and 10(6.5%) (χ2=10.267, P=0.001, OR=3.13, 95%CI=1.514-6.473) in the PD group and control group, respectively. The frequency of the allele A was 160(35.7%) and 70(22.7%) (χ2=14.543, P=0.000, OR=1.889, 95%CI=1.359-2.625) in the PD group and control group, respectively. The frequency of the A53T genotype of A/A was 30(17.4%) and 10(19.2%) in the H-Y≥3 group and H-Y≤2.5 group, respectively, with significant difference (P=0.003, 0.007)as compared to the control group. The frequency of the allele A was 122(35.5%) and 38(36.5%) in the the H-Y≥3 group and H-Y≤2.5 group, respectively, with significant difference (P=0.000, 0.006) as compared to the control group. There was no statistically significant difference of the A53T genotype of A/A and the allele A between the H-Y≥3 group and H-Y≤2.5 group (P=0.768, 0.841). Conclusion The A53T gene polymorphism is the risk factor of Parkinson's disease in Chinese people, but it isn't correlated to stage of sporadic parkinson's disease. Key words: α-synuclein; A53T; Parkinson's disease