Association of A4304G in exon eleven of Brca2 gene with canine mammary tumour

Abstract

Canine mammary tumour is one among the prevalent life style diseases affecting dogs. Certain breeds of dogs are predisposed to mammary neoplasia indicating a possible role of genetic factors in the incidence of disease. Several candidate genes have been studied for its association with mammary tumour in dogs, of which Breast Cancer 2 is one among the genes responsible for the mammary tumour in dogs. In the current study, A4304G within exon 11 of Breast Cancer 2 gene was evaluated for its association with canine mammary tumour. The whole genomic DNA was extracted from 100 female dogs (50 mammary tumour affected dogs and 50 normal animals) above five years. Polymerase Chain Reaction- Restriction Fragment Length Polymorphism (PCR-RFLP) confirmed the presence of the concerned polymorphism among the 100 female dogs under study. On statistical analysis a significant association of the missense variant A4304G with mammary tumour could not be obtained.