Abstract
Objective: To investigate the association between multiple sclerosis (MS) and polymorphisms in intron 8 and exon 9 of the vitamin D receptor ( VDR ) gene. Methods: Blood samples were collected from 100 patients with MS and 100 healthy control individuals in ethylenediaminetetraacetic acid (EDTA) tubes. DNA was extracted and analyzed via polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) for polymorphisms within the VDR. Demographic data were also collected via a questionnaire designed specifically for this study. Results: Our results showed a significant difference between the A/A genotype and between the A and a alleles of intron 8 of VDR ; however, no differences were observed in the other studied alleles. Conclusion: VDR and its functional polymorphisms may be associated with MS. * MS : multiple sclerosis; VDR : vitamin D receptor; MRI : magnetic resonance imaging; CSF : cerebrospinal fluid; EDTA : ethylenediaminetetraacetic acid; PCR-RFLP : polymerase chain reaction–restriction fragment length polymorphism; dNTP : deoxynucleotide triphosphate; dATP : deoxyadenosine triphosphate; dCTP : deoxycytidine triphosphate; dGTP : deoxyguanosine triphosphate; dTTP : thymidine 5′-triphosphate; mRNA : messenger RNA
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