Association of a missense mutation of the &amp;lt;i&amp;gt;MC4R&amp;lt;/i&amp;gt; gene with growth traits in cattle (Brief report) (Assoziation einer Mutation im MC41 Gen mit Wachstumsmerkmalen beim Rind)

C L Zhang,H Chen,L Zhang,R F Zhang,Y H Wang,X Y Lan,A L Zhang

doi:10.5194/aab-49-515-2006

Abstract

Abstract. Melanocortin-4 receptor (MC4R) is one of five G-protein-coupled receptors binding melanocortins that is implicated in the control of ingestive behavior and energy homeostasis. Mutations have been described in the human and mouse MC4R genes which are associated with obesity (YEO et al., 1998; HUSZAR et al., 1997). Moreover, a mutation in porcine MC4R is associated with economically important traits in the pig (KIM et al., 2000). The SNPs reported in bovine MC4R coding region were specific to breeds (VALLE et al., 2004; THUE et al., 2001; HAEGEMAN et al., 2001). In the present experiment over 95% of the coding region of MC4R were screened to detect the SNPs in the predominant cattle breeds of China. Association of a missense mutation of MC4R gene with growth traits was analyzed.

Highlights

Melanocortin-4 receptor (MC4R) is one of five G-protein-coupled receptors binding melanocortins that is implicated in the control of ingestive behavior and energy homeostasis
The previously described 647T>A, 727G>A, 747G>A SNPs were not detected in present populations (VALLE et al, 2004; THUE et al, 2001; HAEGEMAN et al, 2001) confirming the breed-specificity of the polymorphisms
The result indicated that the MC4R genotype was significantly associated with birth weight (P

Summary

Introduction

Melanocortin-4 receptor (MC4R) is one of five G-protein-coupled receptors binding melanocortins that is implicated in the control of ingestive behavior and energy homeostasis. Results: Only two SNPs 927C>T and 1069C>G (published in GenBank #AF265221 correspond to position 653 and 795 in DQ665825, respectively) were detected in 698 unrelated cattle from seven breeds (Nanyang 240, Qinchuan 97, Jinnan 60, Angus 43, Chinese Holstein 61, Luxi 57, Jiaxian 140). The C-to-G (1069) mutation resulted in a valine to leucine substitution. The previously described 647T>A, 727G>A, 747G>A SNPs were not detected in present populations (VALLE et al, 2004; THUE et al, 2001; HAEGEMAN et al, 2001) confirming the breed-specificity of the polymorphisms.

Results

Conclusion