Abstract
BackgroundUpon binding luteinizing hormone in the ovary, the luteinizing hormone/choriogonadotropin receptor (LHCGR) is necessary for follicular maturation and ovulation, as well as luteal function. We detected mutations in the LHCGR gene and evaluated their association with superovulation.MethodsUsing polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and DNA sequencing, we examined polymorphisms in LHCGR and the genotypes associated with superovulation traits in 127 Chinese Holstein heifers.ResultsA G/T polymorphism (ss52050737) in exon 11 was significantly associated with the total number of ova and the number of transferable embryos.ConclusionsLHCGR may be a new predictor for superovulation in Chinese Holstein heifers.
Highlights
Upon binding luteinizing hormone in the ovary, the luteinizing hormone/choriogonadotropin receptor (LHCGR) is necessary for follicular maturation and ovulation, as well as luteal function
The cellular actions of LH are mainly mediated by the luteinizing hormone/choriogonadotropin receptor (LHCGR), which has features typical of receptors that interact with G proteins, including a cellular domain, seven transmembrane domains, and an extracellular hormone-binding domain [6]
Adjustments to superovulation protocols related to luteinizing hormone/choriogonadotropin receptor (LHCGR) availability may increase the yield of bovine embryos [18]
Summary
Upon binding luteinizing hormone in the ovary, the luteinizing hormone/choriogonadotropin receptor (LHCGR) is necessary for follicular maturation and ovulation, as well as luteal function. Great progress has been made toward understanding folliculogenesis and regulation of the hypothalamic-pituitarygonadal axis, and many approaches to superovulatory treatments have been explored, the high variability among individuals in ovarian responsiveness to the application of exogenous hormones remains a challenge. One reason for this variability is that superovulation traits are quantitative and are strongly affected by the environment. Three single-nucleotide polymorphisms (SNPs) in exon 11 of the LHCGR gene are associated with bovine fertility traits [22], and four SNPs of G51656T, A51703G, A51726G and G51737A in intron 9 of the LHCGR gene had significant effects on the total number of ova (TNO) recovered from superovulated Holstein cows [23]. The objective of this study was to investigate the prognostic significance of LHCGR genotypes in superovulation responsiveness
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