ASSOCIATION OF A DISINTEGRIN AND METALLOPROTEASE 33 GENE POLYMORPHISMS AND THEIR HAPLOTYPES WITH ASTHMA IN THE NORTH‑INDIAN POPULATION

Abstract

<p><strong>AIMS, SETTINGS, AND DESIGN:</strong> A disintegrin and metalloprotease 33 (ADAM33), was the first identified asthma‑susceptible gene by positional cloning. A case‑control study was performed. We investigated: (1) Association of ADAM33 gene polymorphisms (V2 C/T, T2 A/G, T1 A/G, Q‑1 A/G, BC+1 A/G and S1 A/G) with asthma and its severity. (2) The distribution of ADAM33 gene polymorphisms’ haplotypes in the studied population and their association with the risk of asthma. <strong>SUBJECTS AND METHODS:</strong> Using polymerase chain reaction and restriction fragment length polymorphism, six polymorphic sites V2 (C/T), T2 (A/G), T1 (A/G), BC+1 (A/G), Q‑1 (A/G), and S1 (A/G) were genotyped in 390 controls and 386 cases of asthma to investigate their association with asthma. Among the recruited cases, 95 (24.6%) were mild intermittent, 235 (60.9%) were mild persistent and 56 (14.5%) were moderate persistent. <strong>STATISTICAL ANALYSIS USED:</strong> The whole analysis was age‑ and gender‑adjusted. Logistic regression model was used to find out the contribution of genetic polymorphisms to the risk of disease. <strong>RESULTS AND CONCLUSION:</strong> We found statistically significant association of single nucleotide polymorphisms (SNPs) T1, S1, and T2 with asthma; however, none of the SNPs were found to be associated with the severity of asthma. GTGGGG haplotype was associated with the risk of asthma (Odds ratio = 4.40; P < 0.0001). In conclusion, results suggest the importance of ADAM33 SNPs with asthma in the North‑Indian population.</p>