Association of β2-adrenergic receptor (β2AR) haplotype and hypertension in African Americans and Caucasians

Abstract

The β2AR gene has been associated with hypertension, but data are conflicting. Racial differences in this association or role of the β2AR haplotype have not been well studied. We sought to determine whether race and/or haplotype influence the association between the β2AR gene and hypertension. Genetic samples were obtained from African American (AA) or Caucasian (C) hypertensive (HT) or normotensive (NT) patients. β2AR codon 16 and 27 genotypes were determined by PCR and luciferase-based pyrosequencing. Differences were analyzed by Chi-square and Fisher's exact test with AA and C analyzed separately. Haplotype frequencies were compared by race between NT and HT. A total of 482 patients were enrolled. Allele and genotype frequencies were similar for HT and NT at codons 16 and 27 for both AA and C. No differences were found in β2AR haplotypes between NT and HT for AA or C (Table). There were no differences in blood pressure across haplotype for NT AA or C. Our data do not support a significant contribution of the β2AR gene to hypertension risk. Clinical Pharmacology & Therapeutics (2004) 75, P95–P95; doi: 10.1016/j.clpt.2003.11.362 Table 1. Haplotype frequencies NT AA (%) HT AA (%) NT C(%) HT C(%) Arg16/Gln27 43 (48.9) 138 (48.0) 96 (38.1) 109 (38.4) Gly16/Gln27 33 (37.5) 90 (31.9) 59 (23.4) 57 (20.1) Gly16/Glu27 12 (13.6) 51 (18.1) 95 (37.7) 118 (41.5) Arg16/Glu27 0 (0) 3 (1.1) 2 (0.8) 0 (0)