Association of -1082 A/G promoter polymorphism of IL-10 gene with colorectal cancer development

Abstract

Event Abstract Back to Event Association of -1082 A/G promoter polymorphism of IL-10 gene with colorectal cancer development Noyko S. Stanilov1*, Zlatka G. Dobreva2, Jovcho Jovchev1, Tashko Deliysky3, Anelia Avramova2, Lyuba Miteva2 and Spaska A. Stanilova2 1 Trakia University, Medical Faculty, Department of Neurosurgery, Surgery and Urology, Bulgaria 2 Trakia University, Medical Faculty, Department of Molecular Biology, Immunology and Medical genetics, Bulgaria 3 University School of Medicine, Oncology Center,, Bulgaria The cancer-associated inflammation and anti-tumor immune response to colorectal carcinoma (CRC) is regulated by cytokines produced from activated immune cells. IL-10 is a Treg cytokine suppressing both Th1 immune response and anti-tumor immunity in hosts. The role of functional polymorphism of IL-10 gene in CRC development still remains elusive. This study was designed to compare -1082 A/G IL10 genotype distribution in 119 CRC patients to a group of 154 matched healthy donors using ARMS-PCR assay. We also investigated serum IL-10 levels in an association to genotype by ELISA. In the study population the slightly enhanced frequency of homozygous genotype GG (18% vs 13%; OR=1.414; 95%CI=0.64÷3.11; p=0.347) was seen in cases versus controls. When CRC patient’s group was divided into stages of disease by TNM classification we observed higher risk of advanced CRC (III-IV stages) for individuals with GG genotype: OR=2.229; 95%CI= 0.828÷6.018; p=0.077. G allele was also overrepresented in advanced vs early CRC (OR=1.581; 95% CI=0.912÷2.743; p=0.082). According to the IL-10 serum levels, CRC patients with GG genotype produced higher IL-10 than AA and AG patients in early stages, similar to healthy control. Moreover, advanced cancer patients with AA and AG genotype produced significantly higher IL-10 compared to patients in early stage (p=0.002 for AA; p= 0.007 for AG genotype), whereas for advanced cancer patients with GG genotype this difference does not reach statistical significance. In conclusion, this study demonstrated that -1082 A/G polymorphism of IL-10 gene has a role in CRC progression rather then CRC genetic predisposition. Keywords: colorectal cancer, IL-10, promoter polymorphism, -1082A>GIL10, Cytokines Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune-mediated disease pathogenesis Citation: Stanilov NS, Dobreva ZG, Jovchev J, Deliysky T, Avramova A, Miteva L and Stanilova SA (2013). Association of -1082 A/G promoter polymorphism of IL-10 gene with colorectal cancer development. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00213 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 14 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Noyko S Stanilov, Trakia University, Medical Faculty, Department of Neurosurgery, Surgery and Urology, Stara Zagora, 6000, Bulgaria, noyko.stanilov@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Noyko S Stanilov Zlatka G Dobreva Jovcho Jovchev Tashko Deliysky Anelia Avramova Lyuba Miteva Spaska A Stanilova Google Noyko S Stanilov Zlatka G Dobreva Jovcho Jovchev Tashko Deliysky Anelia Avramova Lyuba Miteva Spaska A Stanilova Google Scholar Noyko S Stanilov Zlatka G Dobreva Jovcho Jovchev Tashko Deliysky Anelia Avramova Lyuba Miteva Spaska A Stanilova PubMed Noyko S Stanilov Zlatka G Dobreva Jovcho Jovchev Tashko Deliysky Anelia Avramova Lyuba Miteva Spaska A Stanilova Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.