Association interaction and voltammetric determination of 1-aminopyrene and 1-hydroxypyrene at cyclodextrin and DNA based electrochemical sensors

Abstract

The aim of this work was voltammetric determination of 1-aminopyrene and 1-hydroxypyrene using carbon paste electrodes modified with cyclodextrin derivatives and double stranded deoxyribonucleic acid (dsDNA). The detection schemes based on a preconcentration and differential pulse voltammetric (DPV) determination at β-cyclodextrin and γ-cyclodextrin modified carbon paste electrode (β-CD/CPE, γ-CD/CPE), neutral β-cyclodextrin polymer and carboxymethyl-β-cyclodextrin polymer modified screen-printed electrode (β-CDP/SPE, β-CDPA/SPE) and dsDNA modified screen-printed electrode (DNA/SPE) are proposed for the trace determination of studied analytes within the concentration range from 2×10 −8 to 4×10 −7 mol dm −3 and from 2×10 −7 to 4×10 −6 mol dm −3 with the limits of quantification down to 10 −8 mol dm −3. Depending on pH, 1-aminopyrene interacts with both surface attached CD and DNA by electrostatic bonds and supramolecular complexation while 1-hydroxypyrene associates with the CD hosts via complexation. The 1-aminopyrene interaction with dsDNA was confirmed by fluorimetric measurements in the solution phase using a competing DNA–TO-PRO-3 dye complex. In addition, the effect of temperature on this association was investigated using an electrically heated DNA-modified carbon paste electrode (DNA/CPE).