Year-end Sale % OFF 
Abstract
.Divalent ions, in particular calcium ions, constitute important macroelements in living organisms. They are also found in cell membranes, i.e., ensuring their stabilization or participating in synaptic transmission of nerve impulses. The aim of this work is to describe the interactions of divalent ions, such as Ca2+, Ba2+, and Sr2+, in electrolytes with the functional groups on the surface of liposomes formed from phosphatidylcholine (PC). Microelectrophoresis is used to determine the surface charge density as a function of pH. The interactions between ions found in solution and the functional groups of PC are described with the use of a seven-equilibrium mathematical model. Using this model along with experimental data on the charge density of the membrane surface, the association constants characterizing this equilibrium are determined. These parameters are used to calculate the theoretical model curves. The validity of the proposed model is confirmed by comparing the theoretically calculated changes in charge density on the liposome surface with the experimental results.Graphical abstract
Highlights
Every cell of a living organism is enclosed by an integral cell membrane that protects the cell interior from the external environment
We evaluated whether the coverage with Ca2+ and Cl− ions was large enough, in addition to coverage by H+ and OH− ions, to affect the PC membrane surface charge
This paper describes the influence of divalent ions on the surface charge density of the PC liposomal membrane as a function of pH through mathematical equations, with experimental confirmation
Summary
Every cell of a living organism is enclosed by an integral cell membrane that protects the cell interior from the external environment. The surface charge density, which is calculated from the determined electrophoretic mobility, is the parameter that characterizes the liposomal surface. Based on the determined mobility of the PC liposomal membrane, the surface charge density (δ) can be calculated by eq (1). The surface charge density of the lipid membrane results from equilibria existing between the groups localized at the membrane surface and solution ions. By eliminating from eq (20) aA− , using eq (21), the following equation is obtained in the form of a polynomial that can be used to calculate association constants (K4, K5, K6, K7):. The degree of coverage of the liposomal surface occupied by PC with the H+, Me2+, OH−, and Cl− ions was determined from the following equation: Θx ax CPC (26).
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
