Association beween polymorphisms of FAS gene and FAS ligand in cell death pathway with risk of bladder cancer

Abstract

Objective To investigate the associations between the FAS-1377G ＞ A,-670A ＞ G,and FASL-844T ＞ C polymorphisms with risk of bladder cancer.Methods A total of 174 cases of newly diagnosed bladder transitional cell carcinoma and 252 cancer-free controls were enrolled in this study.We genotyped polymorphisms using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).Results We found a statistically significantly increased risk of bladder cancer associated with the FASL-844CC genotype [odds ratio (OR) =1.51 ; 95％ confidence interval (CI) 1.03-2.23].And the FAS haplotype genotypes with 2-4 variant alleles were associated with an increased risk of bladder cancer (OR =2.14; 95％ CI 1.10-4.16).Furthermore,when we evaluated these three polymorphisms together,we found that the combined genotypes with 4-6 variant alleles were associated with an increased risk of bladder cancer (OR =1.58; 95％ CI 1.07-2.34),and this increased risk was more pronounced among subgroups of aged ＞50 years (OR=1.70; 95％CI 1.11-2.61) and smokers (OR=1.88; 95％ CI 1.06-3.32).Conclusion FAS and FASL polymorphisms appear to jointly contribute to risk of bladder cancer in Hubei Han population. Key words: FAS; FAS ligand; Gene polymorphism; Bladder cancer