Association between the rs3733846 in the flanking region of miR-143/145 and risk of cervical squamous cell carcinoma.

Abstract

Aim: To investigate the effect of rs3733846 in the flanking region of miR-143/145 on susceptibility to cervical squamous cell carcinoma (CSCC). Materials & methods: We collected venous blood samples from 242 CSCC patients and 250 healthy controls. The rs3733846 polymorphism was genotyped by SnaPshot and Sanger sequencing. The expression of miR-143/145 in CSCC tissues was detected by quantitative real-time PCR. Results: The rs3733846 AG genotype was associated with a decreased risk of CSCC in genetic model (AGvs.AA: adjustedodds ratio [OR]: 0.44;95% CI: 0.30-0.66;p < 0.001). Patients with the rs3733846 AG/GG genotypes had a reduced risk of developing poorly differential status (OR: 0.57;95% CI: 0.33-0.98;p < 0.04) and lymph node metastasis (OR: 0.49;95% CI: 0.26-0.92;p < 0.03). Conclusion: The rs3733846 in the flanking region of miR-143/145 was related to the susceptibility of CSCC.