Association between the concentration of CYP24A1, 25-OH vit D3 and calcium-phosphate metabolism with an increased risk of multiple sclerosis in Iraqi patients

Abstract

Growing research has shown that multiple sclerosis (MS) patients have lower levels of 25-OHvitD3 than healthy controls. The purpose of this study was to evaluate the serum concentration of 25-OHvitD3, CYP24Alenzyme in MS patients and calcium-phosphate metabolism indices depending on the different disease lines and gender. Furthermore, we elevated the relationship between study parameters and gender with degree of motor disability according to the Expanded Disability Status Scale (EDSS). Serum of 110 Iraqi MS patients (50 male and 60 female) and 63 healthy individuals (30 male and 33 female) as control groups were used in our study. Serum level of 25-OHvitD3, CYP24A1and PTH were measured by ELISA. Serum level for calcium, phosphorus and magnesium were performed with a BioSystems A25 and A15 analyzers. Significantly lower level of CYP24A1 and 25-OHvitD3 was found in MS patients when compared to control groups. The Levels of CYP24A1 and 25-OHvitD3 were positively correlated in female and significantly decreased with EDSS in female groups. Also, in MS patients a highly significant decrease in calcium, a considerable increase in level of PTH, phosphorous and magnesium. In this study the negative correlation between 25-OHvitD3 and the degree of motor disability, according to EDSS in MS patients was confirmed. We identified a non-significant difference in the level of 25-OHvitD3 regarding on gender. Still, we confirmed the association between gender and MS disease development regarding the levels of 25-OHvitD3, PTH and phosphorus.