Abstract

Background: Hepatocellular carcinoma is one of the most fatal malignancies worldwide and is related to many risk factors. Chronic HCV is associated with a 20–30-fold increased risk for HCC. Angiotensin-converting enzyme (ACE) is overexpressed in many cancers and plays a major role in angiogenesis and carcinogenesis. Aim: We aimed to elucidate the effect of the ACE I/D gene polymorphism in patients with HCV-related liver cirrhosis and HCC, and its relationship to clinical parameters. Patients and Methods: The study included 180 participants, cirrhotic (n=60), HCC (n=60) and control healthy subjects (n=60). Liver and renal function tests, alpha-fetoprotein, HCV antibodies and triphasic CT were assessed. ACE Gene polymorphism was assessed by Nested PCR. Results: We observed higher frequencies of DD (36.7%) and DI (51.7%) genotypes, along with the D allele (62.5%), in HCC patients compared to those of cirrhotic cases (10%, 40% and 30%, respectively) and control subjects (6.7%, 38.3%, and 25.8%, respectively). DD and DI genotypes increased the risk and predicted the occurrence of HCC by OR 25.932 and OR 6.354, respectively. The D allele conveys significant risk for HCC compared to control and cirrhotic groups with OR 4.785 and OR 3.889, respectively. Both DD genotype and D allele are significantly correlated with larger tumor size and metastasis. Conclusion: The ACE I/D polymorphism (DD genotype and D allele) is significantly associated with HCC risk in HCV patients and is correlated with increased tumor growth and advanced stage.

Highlights

  • Hepatocellular carcinoma is one of the most fatal malignancies worldwide and is related to many risk factors

  • Aim: We aimed to elucidate the effect of the Angiotensin-converting enzyme (ACE) I/D gene polymorphism in patients with Hepatitis C virus (HCV)-related liver cirrhosis and Hepatocellular carcinoma (HCC), and its relationship to clinical parameters

  • The results of this study demonstrated matched age and gender distributions between the three studied groups, as there was no significant difference in these factors (P= 0.176, p= 0.070 respectively)

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Summary

RESEARCH ARTICLE

Elhamoly1 1Tropical Medicine Department, Faculty of Medicine, Menoufia University, Egypt 2Clinical Pathology Department, Faculty of Medicine, Menoufia University, Egypt 3Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Menoufia University, Egypt 4Clinical Oncology Department, Faculty of Medicine, Menoufia University, Egypt 5Internal Medicine Department, Faculty of Medicine, Al-Azhar University, Cairo, Egypt

Background
INTRODUCTION
Genetic analysis
RESULTS
Significance between groups
Metastasis No Yes
Consensus Guidelines for Hepatocellular
Global Burden of Disease Liver Cancer
Managing Editor
Editorial Board
Full Text
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