Association Between Systemic and Local Oxidative Stress of Infertile Women Undergoing Ivf/Icsi

Qais Ahmed Mahdi,Shatha Abdul Wadood,Rusul H Hamza

doi:10.24996/ijs.2019.60.9.1

Qais Ahmed Mahdi, Shatha Abdul Wadood + Show 1 more

Open Access

https://doi.org/10.24996/ijs.2019.60.9.1

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Abstract

Oxidative stress (OS) may affect in vitro fertilization (IVF) outcomes in infertile women undergoing in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI). The aim of this study is to explore the possible relationships between OS parameters in serum and follicular fluid (FF) from infertile women with male cause infertility (n=40), women with polycystic ovary syndrome (PCOS) (n=40), and women with unexplained infertility (UI)(n=45) undergoing IVF/ICSI. The collection of blood and FF samples was done at the day of oocyte aspiration. Total peroxide (TPX) level, total antioxidant capacity (TAC), and malondialdehyde (MDA) level were measured in serum and FF; whereas, glutathione-s-transferase (GST) activity and superoxide dismutase (SOD) activity were measured in FF. Also, oxidative stress index (OSI) that is the percentage ratio of TPX to TAC, was calculated. In the control group, correlation analysis reveals the presence of a significant positive association between FF OSI with serum OSI, FF TPX with FF OSI, and serum TPX with FF GST activity. In the PCOS group, there was a significant negative association between: FF TPX and serum TAC level. However, non-significant relationship was found between serum and FF OS status parameters in the UI group. Itâ€™s concluded from the present study that systemic OS may give valuable information about local OS occurrence (blood OS reflects FF OS) only in control group and PCOS group. Such information could be useful for a better understanding of the pathological OS mechanisms involved in IVF failure for patients with different causes of infertility.

Highlights

Oxidative stress (OS) is a state caused by an imbalance between Reactive Oxygen Species (ROS)or Reactive Nitrogen Species production and the antioxidant defense systems
0.05) higher compared to control group and unexplained infertility (UI) group; while, non-significant (P 0.05) differences were seen in MDA and total antioxidant capacity (TAC) concentrations among the three groups
The present study showed that MDA levels were higher in serum and follicular fluid (FF) of polycystic ovary syndrome (PCOS) group as compared with control group

Summary

Introduction

Oxidative stress (OS) is a state caused by an imbalance between Reactive Oxygen Species (ROS). An earlier publication had studied total peroxide (TPX) concentrations, total antioxidant capacity (TAC), and oxidative stress index (OSI) in plasma and FF of patients undergoing IVF program [15]. Researchers studied the relationship between ROS, TAC, and redox index in each of plasma FF, and granulosa cell of infertile women undergoing ART procedure [16]. These data lack the simultaneous presence of OS in serum and FF; as well as, the relationship between OS parameters in both of systemic and local levels in regard to different etiological infertile patients. A p value less than 0.05 was considered statistically significant [25]

The OS status parameters in serum and FF

Antioxidant enzymes activity in FF

Association between OS status parameters in UI group