Association between SORT1 gene polymorphism studies in obesity population

Abstract

Obesity is defined the accumulation of extra fat deposited in the body. Based on body mass index (BMI), obesity is classified in children and adults of both men and women. Sortilin 1 (SORT1) gene has been documented with the relationship of low-density lipoprotein cholesterol (LDLC) and coronary artery disease. However, the relation between obesity and LDLC is well documented. The aim of this study was to investigate the relation between rs464218 SNP genotype assay in SORT1 gene among obesity subjects in the Saudi population. One hundred forty samples were categorized as 80 obesity and 60 control subjects. DNA was extracted to perform PCR for rs464218 SNP genotype assay using restriction enzyme. Based on clinical and genotype data, statistical analysis will be performed. The clinical data documents weight, Body Mass Index, High Density Lipoprotein Cholesterol, LDLC and Triglycerides were associated in obese group (p < 0.05). Hardy-Weinberg Equilibrium analysis revealed the deviation with rs464218 SNP genotype assay. None of the genotypes, dominant model and allelic frequencies showed the significant association among obese groups (p > 0.05). Anova analysis revealed the positive association among weight, BMI and LDLC among obese groups (p < 0.05). In conclusion, the rs464218 SNP genotype assay of SORT1 gene doesn’t showed the genotype association but Anova analysis revealed the positive connection between obesity and LDLC. Future studies are required with large number of sample size.