Association between Single Nucleotide Polymorphisms in TYW5 Locus and Beef Amino Acids Content in Shuxuan Cattle

Abstract

Background: Shuxuan cattle is a crossed cattle breed in southwest of China, by crossing the indigenous Bashan cattle with two exotic breeds. The objective of this study was to estimate allele frequencies of the g.88925365A greater than C in TYW5 gene in Shuxuan cattle and to evaluate association between this polymorphism and meat quality traits. Methods: The SNP g.88925365A greater than C was genotyped in 151 Shuxuan cattle and subjected to association analysis with the 17 amino acid content and total amino acid. Result: The AC was the main genotype with 41.06% and C was the predominant allele with 50.99% at this locus. The allele diversity based on the He (0.4998), Ne (1.9992) and PIC (0.3749) revealed moderate genetic variation within Shuxuan cattle population. Significant association (P less than 0.05) was observed between the polymorphism and six amino acid content (Glu, Asp, Val, Ile, Ser and Cys). These results provided a potential genetic marker for the genetic improvement of meat quality in Shuxuan cattle.