Abstract
BackgroundThe disease progression following hepatitis B virus (HBV) infection is associated with single-nucleotide polymorphisms (SNPs). However, the role of SNPs in chronic HBV infection in children remains unclear. Here, we investigate the association between SNPs and early spontaneous hepatitis B e antigen (HBeAg) seroconversion in children with chronic hepatitis B infection.MethodsThis was a retrospective cohort study. We genotyped seven SNPs in the following genes, interleukin (IL)-10 (rs1800871 and rs1800872), human leukocyte antigen (HLA)-DPA1 (rs3077), HLA-DPB1 (rs9277535), HLA-DQB2 (rs7453920), HLA-DQB1 (rs2856718), and IL28B (rs8099917), in patients with chronic HBV infection using PCR and sequencing. These variants were analyzed for an association with early HBeAg seroconversion in children.ResultsOf 225 Japanese patients with chronic hepatitis B virus infection (male/female: 105/120, median age at initial visit: 6 years; range 0–44 years), 52 achieved spontaneous HBeAg seroconversion at the age of 10 years or younger (G1: early seroconversion group), and 57 did not achieve spontaneous HBeAg seroconversion under the age of 20 years (G2: late or no seroconversion group). Of the seven SNPs, only the HLA-DPA1 SNP displayed a low p-value (P = 0.070), but not significant, to have early HBeAg seroconversion in the dominant model and in the allele model (P = 0.073) using the chi-square test. The association study found a low p-value, but not significant, to have early HBeAg seroconversion in the dominant model for HLA-DPA1 (genotype TC + TT vs. CC, P = 0.070, odds ratio: 2.016, 95% confidence interval: 0.940-4.323) using a logistic regression model.ConclusionAlthough the HLA-DPA1 SNP did not show a statistically significant association with early HBeAg seroconversion in this study, the HLA-DPA1 SNP might increase the likelihood of achieving early spontaneous HBeAg seroconversion in children.
Highlights
The disease progression following hepatitis B virus (HBV) infection is associated with single-nucleotide polymorphisms (SNPs)
Recent genome-wide association studies showed that genetic variants of human leukocyte antigen (HLA)-DP and HLA-DQ are strongly associated with the outcome of HBV infection in adults from Japan, Korea, and China [12,13,14,15,16,17,18,19,20,21]
We retrospectively evaluated the effect of genetic variants of IL-10, HLA-DP, HLA-DQ, and IL-28B on early spontaneous hepatitis B e antigen (HBeAg) seroconversion in children with chronic HBV infection
Summary
The disease progression following hepatitis B virus (HBV) infection is associated with single-nucleotide polymorphisms (SNPs). We investigate the association between SNPs and early spontaneous hepatitis B e antigen (HBeAg) seroconversion in children with chronic hepatitis B infection. Single nucleotide polymorphisms (SNPs) are well known to affect disease progression following HBV infection. Recent genome-wide association studies showed that genetic variants of human leukocyte antigen (HLA)-DP and HLA-DQ are strongly associated with the outcome of HBV infection in adults from Japan, Korea, and China [12,13,14,15,16,17,18,19,20,21]. We retrospectively evaluated the effect of genetic variants of IL-10, HLA-DP, HLA-DQ, and IL-28B on early spontaneous hepatitis B e antigen (HBeAg) seroconversion in children with chronic HBV infection
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
