[Association between single nucleotide polymorphism of macrophage migration inhibitory factor-rs1007888 and the pathogenesis of gestational diabetes mellitus].

Abstract

To investigate the association between single nucleotide polymorphism (SNP) of macrophage migration inhibitory factor (MIF) gene-rs1007888 and the pathogenesis of gestational diabetes mellitus (GDM). A total of 120 GDM pregnant women (GDM group) and 165 healthy pregnant women (control group) from Affiliated Hospital of Medical College, Qingdao University were recruited from June 2011 to July 2012. Their age, gestational week, height and weight were recorded. The levels of fasting blood glucose (FBG) and fasting insulin (FIN) were determined. Body mass index (BMI), the hemeostasis model assessment-insulin resistance (HOMA-IR) and hemeostasis model assessment-β cell function (HOMA-β) were calculated. DNA was extracted from fasting blood samples. SNP of MIF-rs1007888G/A was determined by DNA sequencing.The FBG, FIN, HOMA-IR and HOMA-β were compared between GDM group and the control group.They were also compared among pregnancies with different genotypes. (1) GDM group had higher FBG, FIN and HOMA-IR levels, but lower HOMA-β than the control group (all P < 0.05). (2) MIF-rs1007888 SNP genotype frequencies of GG, GA and AA were 37.5%, 45.8% and 16.7%, and the allelic frequencies of G and A were 60.4%, 39.6% in GDM group; However, in the control group, the frequencies of GG, GA and AA were 26.1%, 54.5% and 19.4%, and the allelic frequencies of G and A were 53.3%, 46.7%, respectively.The distributions of MIF genotypes in GDM patients were significantly different from the healthy subjects (P < 0.05).No significant difference of MIF-rs1007888 allele distributions was observed between GDM group and the control group (P > 0.05). (3) The FBG, FIN and HOMA-IR in pregnant women with GG genotype were statistically higher than those with GA or AA genotypes, while HOMA-β was lower in women with GG genotype (all P < 0.05). The SNP of MIF rs-1007888 was related to the insulin resistance and pancreatic β cell function of pregnant women. GG genotype of MIF-rs1007888 might be a genetic susceptible factor in the pathogenesis of GDM.