Abstract
Several studies examined the impact of miR-34b/c rs4938723 polymorphism and cancer risk, but the findings are inconsistent. However, no study has been conducted to inspect the impact of miR-34b/c polymorphism on bladder cancer. This study aimed to assess possible association between rs4938723 polymorphism and bladder cancer risk. This case-control study was done on 136 pathologically proven bladder cancer patients and 144 controls. Genotyping of Pri-miR-34b/c rs4938723 polymorphism was achieved by using the polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method. Our findings did not show any statistically significant differences in genotype and allele frequencies between bladder cancer and controls. Larger sample sizes with diverse ethnicities are required to validate our findings.
Highlights
Bladder cancer is the ninth most common malignancy in the world, and the fourth most common cancer in the United States[1]
The miR-34b/c rs4938723 t > C polymorphism was successfully genotyped for 136 bladder cancer patients (117 males, 19 females) with an average age of (63.8Æ12.3) years and 144 controls (134 males, 10 females) with mean age of (64.3Æ10.2) years
The results indicated that pri-miR-34b/c rs4938723 t > C polymorphism was not associated with the risk of bladder cancer in codominant (OR = 1.08, 95% confidence interval (CI) = 0.66–1.78, P = 0.800; Odds ratio (OR) = 2.13. 95%CI = 0.91–5.01, P = 0.094, TC vs. TT), dominant (OR = 1.22, 95%CI = 0.76–1.95, P = 0.468, TC + CC vs. TT), recessive (OR = 2.04, 95%CI = 0.91–4.60, P = 0.110, CC vs. TT + TC), overdominant (OR = 0.94, 95%CI = 0.59–1.50, P = 0.812, TC vs. TT + CC) and allelic (OR = 1.28, 95%CI = 0.90–1.82, P = 0.181, C vs. T) inheritance model tested
Summary
Bladder cancer is the ninth most common malignancy in the world, and the fourth most common cancer in the United States[1]. Men are more than four times more likely to get bladder cancer than women. It has been proposed that the development of bladder cancer is a result of environmental factors such as smoking, occupational exposure to carcinogens, obesity, physical inactivity[2–4], genetic variants and the interaction of genes with the external factors[5–8]. MicroRNAs (miRNAs) are a class of small singlestranded noncoding RNA molecules that play key roles in a variety of cellular processes by targeting mRNAs for cleavage or translational repression[9–10]. The data provides strong evidence that dysregulation of miRNAs expression affects the tumorigenesis by acting as oncogenes or tumor suppressors[11–15]. Single-nucleotide polymorphisms (SNPs) in miRNAs can affect cancer susceptibility by disturbing miRNAs biosynthesis and expression, altering mature miRNAs, or by combining with target genes[16–19]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
