ASSOCIATION BETWEEN POLYMORPHISMS IN CYTOKINE IL7 GENE AND CMV IN RENAL FAILURE PATIENTS IN DIYALA PROVINCE

Abstract

Background: The human cytomegalovirus (HCMV) is an important human pathogen primarily affecting immunocompromised patients, like Renal failure patients, transplant recipients or HIV-infected individuals. Renal failure diesease is a wide dissemination among kidney patients in Ba'quba City. Aim: the study was carried out to detect the HCMV and detection of several single nucleotide polymorphisms (SNP) in IL7 among100 patient suffering from acute or chronic renal failure whom admitted to Ibn Sina Center for kidney Dialysis in Baquba Teaching Hospital. Patients and Methods: This study was conducted for the period from 1/12/2019 to 15/6/2020 in Baquba city in Iraq, with age ranged between (13-76) years. The samples were diagnosed by serological and molecular tests. Results: The obtained results showed that HCMV antibody was detected in renal failure patients by ELISA IgG (100%) while IgM were (15.0%) . Also, Distribution of genotypes for the (IL7) rs7007634 gene samples of renal failure patients 15 out of 20, Chi-Square (9.375) investigated samples (P value 0.002) . The alignment results of the 323 bp samples revealed the presence of one genetic variation variably distributed in some of the analyzed samples in comparison with the referring IL7 genetic sequences. Distribution of genotypes of the rs7007634(IL7)gene in renal failure patient samples according to CMV infection 12 sample infection in CMV and 3 sample without CMV.