Association between Platelet-Specific Collagen Receptor Glycoprotein 6 Gene Variants, Selected Biomarkers, and Recurrent Pregnancy Loss in Korean Women.

Hui Jeong An,Sung Hwan Cho,Jung Ryeol Lee,Chang Soo Ryu,Ji Hyang Kim,Jung Oh Kim,Young Ran Kim,Nam Keun Kim,Han Sung Park,Woo Sik Lee,Eun Hee Ahn

doi:10.3390/genes11080862

Abstract

This paper investigates whether glycoprotein 6 (GP6) gene polymorphisms are a risk factor for recurrent pregnancy loss (RPL) in Korean women. Genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism and real-time polymerase chain reaction amplification. We identified five polymorphisms in the GP6 gene: rs1654410 T>C, rs1671153 T>G, rs1654419 G>A, rs12610286 A>G, and rs1654431 G>A. GP6 rs1654410 CC was associated with decreased RPL risk (adjusted odds ratio = 0.292, 95% confidence interval = 0.105–0.815, p = 0.019), and recessive genotypes were also significantly associated with decreased RPL risk (adjusted odds ratio = 0.348, 95% confidence interval = 0.128−0.944, p = 0.038). GP6 rs1654419 GA was associated with decreased RPL risk (adjusted odds ratio = 0.607, 95% confidence interval = 0.375-0.982, p = 0.042), and dominant genotypes were significantly associated with decreased RPL risk (adjusted odds ratio = 0.563, 95% confidence interval = 0.358−0.885, p = 0.013). Altogether, the genotype frequencies of GP6 rs1654410 T>C and GP6 rs1654419 G>A were significantly different between RPL patients and control participants. Therefore, although GP6 polymorphisms may be useful as biomarkers of RPL, additional studies with heterogeneous cohorts are required to better understand the influence of GP6 and assess its performance as a biomarker.

Highlights

Recurrent pregnancy loss (RPL) is defined as two or more consecutive pregnancy losses [1]
We found a significant association between glycoprotein 6 (GP6) polymorphisms and three or more recurrent pregnancy loss (RPL): GP6 rs1654410 T>C
Our results indicate that several polymorphisms are associated with clinical variables in RPL patients: GP6 rs1671153 T>G, rs1654419 G>A, and rs12610286 A>G were associated with higher homocysteine levels, elevated creatinine levels, and Plasminogen Activator Inhibitor-1 (PAI-1), respectively

Summary

Introduction

Recurrent pregnancy loss (RPL) is defined as two or more consecutive pregnancy losses [1]. The likelihood of pregnancy loss is 5% higher for women. Genes 2020, 11, 862 who suffer a miscarriage during their first pregnancy than for healthy subjects [3]. Many factors that contribute to the etiology of RPL have been identified, the underlying cause remains unknown in the majority of cases. RPL is associated with immune disorders, blood coagulation, and angiogenesis. These factors are related to hemostasis, which in turn contributes to platelet activation. Collagen is a platelet activator that plays an important role in the vascular endothelium and vascular wall. Platelets have two major receptors for collagen: integrins, which play a role in platelet aggregation, and glycoprotein 6

Objectives

Methods

Results

Discussion

Conclusion