Association between paraoxonase-1 gene promoter -108C/T polymorphism and myocardial infarction in the Tunisian male population / Tunuslu erkek populasyonunda miyokard enfarktüs ile paraoksonaz -1 gen başlatıcı -108C/T polimorfizmi arasındaki ilişki

Abstract

Abstract Objective: Human paraoxonase 1 (PON1) is an HDL-associated enzyme with anti-oxidant/anti-inflammatory properties that has been suggested to play an important protective role against coronary artery disease (CAD). The PON1 promoter -108C/T polymorphism has been analyzed in numerous association studies as a genetic marker for CAD, however, with controversial results. The aim of this study was to evaluate the association of PON-1 promoter -108C/T polymorphism with the risk of myocardial infarction (MI) in the Tunisian male population. Methods: A total of 815 subjects were recruited, including 318 healthy controls and 497 MI patients. Genotypes were determined by PCR-RFLP method. Genotype/allele frequencies were compared in patients and controls using the chi-square test. Results: Genotype distributions and allele frequencies of PON-1 promoter -108C/T polymorphism were different among the control and MI groups. Patients with MI had significantly higher frequency of the TT genotype compared to controls [29.2% vs. 25.5%; OR (95% CI), 1.67 (1.52-2.49); p=0.010]. The MI patient group showed a significant higher frequency of the T allele compared to the controls [0.56 vs. 0.51; χ2=8.61, p=0.013]. The association between the PON-1 promoter -108C/T polymorphism and MI remained significant after adjustment for other well-established cardiovascular risk factors. Conclusion: The present study showed a significant and independent association between the PON-1 promoter -108C/T polymorphism and MI in the Tunisian male population.