Association between Monocyte Chemoattractant Protein-1 -2518 (A/G) Single Nucleotide Polymorphism and Chronic Periodontitis in End-stage Renal Disease Patients – A Case–control Study

Abstract

ABSTRACT Monocyte chemoattractant protein 1 (MCP-1) plays an important role in the development of periodontitis. The purpose of this investigation was to evaluate association of the MCP-1 gene polymorphism with chronic periodontitis in patients with end-stage renal disease (ESRD). One hundred fifty ESRD patients with chronic periodontitis (CP), 100 without CP and 190 healthy controls were included in this study. Genomic DNA from all participants was genotyped for the –2518 (A/G) polymorphism by a polymerase chain reaction – restriction fragment length polymorphism (PCR­-RFLP) assay. Significant differences were observed in the genotype and allele frequencies between patients with ESRD and CP and controls. The G allele frequency was significantly higher in patients than in control subjects, with odds ratio 1.77 (95 % CI 1.2–2.5), p = 0.0014. For the GG genotype the OR was 3.63 (95 % CI 1.5–8.76), p = 0.041. No significant differences in the polymorphism distribution were observed between ESRD patients without CP and control subjects. Comparison of the MCP-1 gene polymorphism distribution in ESRD patients with various primary diseases leading to ESRD did not show any significant differences. The mean MCP-1 serum levels were compared between subgroups. They were significantly higher in ESRD patients with CP (582 ± 112 pg/ml) than in patients without CP (309 ± 103 pg/ml) and controls (265 ± 85 pg/ml). Our results suggest that the MCP-1-2518 A/G polymorphism might be a novel risk factor for developing chronic periodontitis in patients with ESRD.