Association between lncRNA IFNG-AS1 SNP and Hashimoto′s thyroiditis

Abstract

Objective To assess the association between three single nucleotide polymorphisms(SNPs)(rs10878724、rs7980829 and rs11177020) of lnc IFNG-AS1 and Hashimoto′s thyroiditis(HT) susceptibility. Methods TaqMan probe technology was used to genotype the selected SNPs in a total of 179 subjects, including 70 HT cases, and 109 controls. The expression levels of lnc IFNG-AS1 and IFNG were detected by SYBR-Green qRT-PCR. Results Compared with control, not only the A allele and AA genotype frequencies of rs10878724 were significantly different in group HT(P=0.01, P=0.003), but also the T allele and TT genotype frequencies of rs7980829 were significantly high in group HT. Haplotype analysis showed that the G-G-A decreased the risk of HT(P=0.04), while the haplotype of A-T-T incresed the risk of HT(P=0.01). The relative expression of both IFNG mRNA and lnc IFNG-AS1 were higher in group HT than in control(P=0.001, P=0.013). In HT patients, IFNG mRNA relative expression in both rs7980829-TT and rs1087872-TT were significantly higher than those of other genotypes(P=0.017, P=0.009). Conclusion The SNPs of Inc IFNG-AS1 were correlated with the expression levels of IFNG and lncRNA IFNG-AS1. Noncoding genes should be further screened as potential biomarkers in prediction of HT susceptibility. Key words: IFNG gene; IFNG-AS1; Long non-coding RNA; Single nucleotide polymorphisms; Hashimoto thyroiditis