Association between IL1B gene and cervical cancer susceptibility in Chinese Uygur Population: A Case–Control study

Li Wang,Jiajing Hong,Fanglin Niu,Shanshan Zhang,Jing Li,Tianbo Jin,Wenhui Zhao

doi:10.1002/mgg3.779

Abstract

BackgroundInterleukin‐1β (IL‐1B) has been recognized as a pro‐inflammatory cytokine and associated with tumorigenesis. We aimed to evaluate the contribution of IL‐1B polymorphisms to the susceptibility of cervical cancer in Chinese Uygur population.MethodsSeven variants were genotyped by Agena MassARRAY platform in 267 cervical cancer patients and 302 healthy controls. Allelic, genotypic, and haplotypic association analyses adjusted for age were investigated using odds ratios (OR) and 95% confidence intervals (CI). GEPIA and UALCAN databases were used to evaluate expression and prognostic of IL‐1B gene in cervical cancer.ResultsOur result revealed IL‐1B rs1143627‐AA (OR = 1.98, p = 0.029) and rs16944‐GG (OR = 2.01, p = 0.025) was associated with an increased risk of cervical cancer. Besides, we also found two protective single nucleotide polymorphisms (SNPs) rs3136558 (OR = 0.63, p = 0.011) and rs1143630 (OR = 0.63, p = 0.019). Haplotype ″TGA″ in the block (rs1143630, rs1143627, and rs16944) significantly decreased the susceptibility of cervical cancer (OR = 0.53, p = 0.0007). IL‐1B mRNA level was up‐regulated in the cervical cancer patients, which was related with poor prognosis in silico.ConclusionsFor the first time, our results provide evidence on polymorphism of IL‐1B gene associated with cervical cancer risk in Chinese Uygur population.

Highlights

Cervical cancer is considered to be the second most common gynecologic tumor after breast cancer and one of the main leading causes of cancer‐related mortality among women worldwide
Previous studies have reported that many genetic variations in immune‐associated genes including tumor necrosis factor, interleukin (IL), and DNA repair genes may play a crucial role in the cervical carcinogenesis risk (Pontillo et al, 2016)
By HaploReg annotation, we found that the selected single nucleotide polymorphisms (SNPs) were associated with regulation of promoter and/or enhancer histone, DNase, proteins bound, motifs changed, selected eQTL hits

Summary

Introduction

Cervical cancer is considered to be the second most common gynecologic tumor after breast cancer and one of the main leading causes of cancer‐related mortality among women worldwide. More than 100,000 new cervical cancer cases are diagnosed in China every year, and many individuals have not yet been diagnosed (Chen et al, 2016). It is reported that several interleukin gene polymorphisms (such as IL‐1, IL‐6, and IL‐10) are associated with risk of cervical cancer (Guo et al, 2018; Zidi et al, 2015, 2017). We aimed to evaluate the contribution of IL‐1B polymorphisms to the susceptibility of cervical cancer in Chinese Uygur population. Conclusions: For the first time, our results provide evidence on polymorphism of IL‐1B gene associated with cervical cancer risk in Chinese Uygur population

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