Association Between HLA-DQ Genotypes and Haplotypes vs Helicobacter pylori Infection in an Indonesian Population

Yang Zhao,Shinkan Tokudome,Jingwen Wang,F.X Ediati Triningsih,Tegu Triono,Akihiro Hosono,Tsutomu Tanaka,Soeripto Soeripto,Ryosuke Ando,Suwignyo Sumoharjo,E.Y Wenny Astuti,Stephanus Gunawan

doi:10.7314/apjcp.2012.13.4.1247

Abstract

Helicobacter pylori is an important gastrointestinal pathogen related to the development of not only atrophic gastritis and peptic ulcer, but also gastric cancer. Human leukocyte antigens (HLA) may play particular roles in host immune responses to bacterial antigens. This study aimed to investigate the association between HLA-DQA1 and DQB1 genotypes and haplotypes vs H. pylori infection in an Indonesian population. We selected 294 healthy participants in Mataram, Lombok Island, Indonesia. H. pylori infection was determined by urea breath test (UBT). We analyzed HLA-DQA1 and DQB1 genotypes by PCR-RFLP and constructed haplotypes of HLA-DQA1 and DQB1 genes. Multiple comparisons were conducted according to the Bonferroni method. The H. pylori infection rate was 11.2% in this Indonesian population. The DQB1*0401 genotype was noted to be associated with a high risk of H. pylori infection, compared with the DQB1*0301 genotype. None of the HLA-DQA1 or DQB1 haplotypes were related to the risk of H. pylori infection. The study suggests that HLADQB1 genes play important roles in H. pylori infection, but there was no statistically significant association between HLA-DQA1 or DQB1 haplotypes and H.pylori infection in our Lombok Indonesian population.

Highlights

This study aimed to investigate the association between Human leukocyte antigens (HLA)-DQA1 and DQB1 genotypes and haplotypes vs H. pylori infection in an Indonesian population
No obvious differences were noted for H. pylori infection rates by sex or age
Individuals carrying DQB1*0401 genotypes were noted to be at higher risk of H. pylori infection, compared with those carrying DQB1*0301 (OR=5.24, 95% CI=1.25-21.91) (Table 2)

Summary

Introduction

More than 50% of the world’s population is infected with Helicobacter pylori (H. pylori) in the pylorus of the stomach (Brown, 2000). H. pylori is an important gastrointestinal pathogen associated with chronic gastritis, gastric or duodenal ulcers, and gastric cancer (Asaka et al, 1994; Honda et al, 1998; Peek et al, 2000 ). Host individual differences in immune response have appeared to play a key role in the establishment and progression of H. pylori infection. Helicobacter pylori is an important gastrointestinal pathogen related to the development of atrophic gastritis and peptic ulcer, and gastric cancer. This study aimed to investigate the association between HLA-DQA1 and DQB1 genotypes and haplotypes vs H. pylori infection in an Indonesian population. Conclusions: The study suggests that HLADQB1 genes play important roles in H. pylori infection, but there was no statistically significant association between HLA-DQA1 or DQB1 haplotypes and H.pylori infection in our Lombok Indonesian population

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Results