Abstract
The aim of the experiment was to detect polymorphism in the GPX5 gene and to determine associations between individual genotypes and selenium concentration in liver and kidney of wild boars from West Pomerania province in Poland. The polymorphism in GPX5 gene was detected using the PCR-RFLP method with specific primers and the restriction enzyme Hinf I. Two different alleles of the GPX5 gene were identified - allele 1B (0.37) and 2B (0.63). The relationships between the GPX5 genotypes and concentrations of selenium in liver and kidney were analyzed, revealing statistically significant (P≤0.05) differences between wild boars carrying different GPX5 genotypes in both genders. Obtained results indicated that wild boars with the 2B2B genotype had the highest selenium concentration in livers and kidneys compared to 1B1B and 1B2B animals and these differences were statistically significant (P≤0.05).
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