Association between genetic variations in LIN28/let-7 pathway and Wilms tumor susceptibility

Abstract

To investigate the correlation between genetic variants of key genes in LIN28/let-7 pathway and the risk of Wilms tumor in Chinese population. We performed a four-centre hospital-based case-control study including 355 Wilms tumor cases and 1070 controls with the purpose to determine whether the potentially functional single nucleotide polymorphisms in LIN28/let-7 pathway core genes are associated with Wilms tumor risk in Chinese children. DNA was extracted and genotyping was performed using TaqMan assays. Genotype frequencies of the polymorphisms and the demographic variables between Wilms tumor cases and controls were compared using the chi-squared test. The associations between the selected polymorphisms and Wilms tumor risk were measured by calculating the odds ratios (ORs) and 95% confidence intervals (CIs), using unconditional univariate and multivariate logistic regression analyses. Stratified analysis was used to identify the association between genotypes in subgroups and the risk of Wilms tumor. 1.LIN28A rs3811463 T>C and rs34787247 G>A were associated with increased risk of Wilms tumor.2.LIN28B rs314276 C>A was associated with decreased risk of Wilms tumor.3.Individuals harboring KRAS rs12587 GT genotype were more likely to develop Wilms tumor than those carrying the GG genotype.4.No associations with risk of Wilms tumor were found for the selected SNPs of the HMGA2 gene.5.No associations with risk of Wilms tumor were found for the selected SNPs of the CYMC gene. The LIN28A rs3811463 T>C and rs34787247 G>A, LIN28B rs314276 C>A and KRAS rs12587 G>T may contribute to risk of Wilms tumor in Chinese population. More centres, larger, prospective studies with different ethnic populations are warranted to validate our findings.