Association between Genetic Polymorphism of 5-HTTLPR, and SGOT, SGPT and Catalase with Alcoholism of Iraqi People

Abstract

A case- control study on the relationship of 5-HTTLPR gene polymorphism with alcohol abuse among Iraqi individuals was conducted during period from December 2018 to June 2019. DNA was extracted from the blood samples of the study subjects. The genetic polymorphism analysis was conducted by Restriction Fragment Length Polymorphism (RFLP-PCR) for SLC6A4 gene. The results raveled that the odd ratio for the LL genotype was 1.697 indicating that homo mutant genotype were at higher risk for alcoholism than the wild type SS. This result showed that L allele frequency was (0.78) in alcoholism group and (0.53) in control group whereas S allele frequency was (0.22) in the alcoholism group and (0.47) in the control group. So, the results showed there was a significant difference in allele frequencies of SLC6A4gene between case and control (P<0.01; OR=1.697; 95% CI=0.86-1.64). There was a significant positive correlation between the SGOT activity and the genotype of 5-HTTLPR (SS, SL, LL).Additionally, there was a significantly increased level of liver enzymes (GOT and GPT) and catalase in serum samples of alcoholic males compared with non-alcoholic males.