Association between gene polymorphism of the tissue inhibitor matrix metalloproteinase-2 (G303→A) and enterocutaneous fistula

Abstract

Objective – to analyze the frequency of polymorphic variants of tissue inhibitors of metalloproteinase-2 (G303→A) gene in patients with enterocutaneous fistula. Material and methods. The object of the study comprises 19 patients with enterocutaneous fistula who were treated in the Shalimov National Institute of Surgery and Transplantology during 2016-2019. Laboratory, genetic, histological studies and statistical analysis were performed.Results. As a result of genetic and statistical analysis of the tissue inhibitors of metalloproteinase-2 (G303→A) gene single nucleotide polymorphisms, genotype variants have been identified that are associated with the risk of enterocutaneous fistula development. All models of inheritance were analyzed and the best model with the lowest Akaike information criterion, which turned out to be a recessive model, has been determined.Conclusions. Enterocutaneous fistula is 1,58 times more common in carriers of homozygous GG genotype of the tissue inhibitors of metalloproteinase-2 (G303→A) gene and twice less common in heterozygotes GA (21,1% vs. 40%, p=0,057). Carriers of minor homozygotes of AA genotype in the group with enterocutaneous fistula were not detected, while a similar genotype in the control group was found in 10% of cases.