Association between gene polymorphism of growth hormone and carcass traits in dairy bulls

Abstract

AbstractThe contribution of the leucine/valine substitution at amino acid position 127 in the bovine growth hormone (GH) protein to variation in carcass traits was studied. The data included 109 Polish Friesian bulls slaughtered at 15 months of age. The traits measured were carcass gain, weights of meat, bones, intermuscular and subcutaneous fat in the carcass and meat, bones and fat in valuable cuts (fore and best ribs, sirloin, round of beef and shoulder). The bulls’ GH genotype was determined using the PCR-RFLP technique. The frequencies of leucine (Leu) and valine (Val) alleles were 0·64 and 0·36, respectively. The GH concentration was determined in serial blood plasma samples collected every 15 min starting from 15 min before to 135 min after intravenous administration of 0·15 µg thyrotropin releasing hormone (TRH) per kg live weight. Response GH variables were: baseline (the mean of samples collected at –15 and 0 min), peak (the sample taken at 15 min post injection of TRH) and disappearance rate (calculated as peak minus the sample at 60 min, divided by time interval 45 min). Mixed animal models were used for the statistical analysis. Differences were found between the Leu/Leu and the Val/Val genotypes for carcass gain and weight of meat in the carcass (P≤ 0·05). Moreover, differences in the size of the GH peak between the two homozygotes approached significance (P≤ 0·10). The effect of GH genotype accounted for a moderate part of the phenotypic variance in the carcass traits, corresponding to a reduction in the residual variance of ≤ 5·25% when included in the model, whereas the corresponding value for the effect of GH genotype on the variation in GH release was lower, ≤ 1·77%. In conclusion, the Leu/Val polymorphism seems to be associated with carcass traits in dairy bulls, although the effect was relatively small when compared with the effects of season and background genome.