Abstract
The Fbxl5 gene is a member of the F-BOX family and plays an important role in maintaining iron homeostasis in cells. In order to reveal the genetic effects of Fbxl5 gene polymorphisms on body weight (BW) traits and reproductive performance in chickens, Fbxl5 gene polymorphisms were detected in 363 Jinghai Yellow chickens by PCR-single-strand conformation polymorphism (SSCP) and DNA sequencing methods using three primers. With primer 1, three genotypes (BB, bb, Bb) were detected in the Jinghai Yellow chicken population and two mutations (g. 14257 T C and g. 14262 T C) were revealed by gene sequencing. With primer 2, two genotypes (EE, Ee) were detected in the same population and one mutation (g. 19018 G A), and for primer 3, three genotypes (FF, ff, Ff) and one mutation (g. 19018 G A) were detected. Four single nucleotide polymorphisms (SNPs) were used to estimate the frequency distributions of the eight haplotypes with PHASE 2.1 software. CTCG was the major haplotype with a frequency of 37.93 %, while the least frequent was TCTA with a frequency of 2.98 %. The BW of haplotype combination H1H8 was higher than that of the other haplotypes and was a dominant combination. In terms of reproductive performance, the age at the first egg of the haplotype combination H9H1 was later than in the other haplotypes, but the mean egg weight at 300 days was relatively optimal. The H1H2 haplotype produced the highest mean egg weight in 300 days, although the total number of eggs in 300 days was smaller in the H2H4 haplotype with the highest at first egg. Therefore, we can consider using the haplotype combination H1H2 for selection. The findings of this study expand the theoretical basis of the use of the Fbxl5 gene in the molecular breeding of poultry.
Highlights
F-box and leucine-rich repeat protein 5 was discovered by Ruiz et al (2014) using a database search method
Using specific-locus amplified fragment sequencing (SLAF-seq) and a genome-wide association study (GWAS), we identified 19 single nucleotide polymorphism (SNP) loci that were significantly related to the growth of Jinghai Yellow chickens and mapped them to nine genes
Single-strand conformation polymorphism (SSCP) analysis revealed that the products of the three primer pairs displayed polymorphisms in the Fbxl5 gene
Summary
F-box and leucine-rich repeat protein 5 was discovered by Ruiz et al (2014) using a database search method. Different ubiquitinated enzymes target different substrate proteins and determine the specificity of ubiquitination, so that related proteins can be degraded (Darosa et al, 2015). It is closely involved in the cell cycle, signal transduction, DNA repair, immune response, and DNA regulation (He et al, 2016). High expression of Fbxl contributes to the occurrence and development of lung cancer They found that there was a negative correlation between the protein levels of HSSBI and Fbxl. The Fbxl gene targets HSSBI to mediate its ubiquitination and subsequent degradation, and to regulate the repair of DNA damage (Chen et al, 2014). Other studies had shown that the primary role of the Fbxl gene was to mediate the subsequent interpretation of the ubiquitin iron regulatory proteins IRP1 and IRP2 through iron dependence, which plays a crucial role in maintaining iron homeostasis in cells (Ruiz et al, 2014)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
