Association between Effect Ofsome Pro-inflammatory and Anti-inflammatory Cytokines before and After Surgical Patients

Abstract

The aims of our study were to investigate association of some effect of pro- inflammatory and antiinflammatory cytokines before and after surgical patients through study serum concentration of, TNF-?,IL-2, IL-4, IL-6, IL-10. 35 blood samples were collected from surgical patients (17 male, 18 female)withgeneral anesthesia from Al-Diwaniyah Hospital, Iraq. Samples were taken before surgery (Tpre), the firstday) T1), third day) T3 (and fifth day (T5) after operative after skin incision.Pro-inflammatory cytokines(IL-2, IL-6, and TNF-?) and anti-inflammatory cytokines (IL-4, IL-10) were quantitatively analyzed byELISA technique.Our resultsshowed there were no significant difference (p<0.05) in WBCs count, WBCspercentage (neutrophil, eosinophil, basophil, monocytes and lymphocytes), PCV, PCT percentage and HBconcentration before (Tpre) and after (T1, T3, and T5) surgical operation. Also the results showed therewere a significant decrease in IL-2 concentration in (T1) and (T3) day postoperative surgery comparedwith (T pre), and returned to the pre-operative values on (T5). IL-6 showed significant increase in first daypostoperative (T1), and then decreased the (T3 and T5) compared to the (Tpre), TNF? slightly increasedin (T1 and T5) but the difference didn’t significantly, IL-4 didn’t show any significant difference betweenTpre, T1, T3 and T5. IL-10 showed a significant increase in (T1) compared with (Tpre), and returned tothe preoperative values on (T3) and (T5). We could be concludethe pro? and anti?inflammatory cytokinesbalanced ratio is essential to regulate the inflammatory responses postoperative. Anti-inflammatory cytokine(IL-10) increased in first day postoperative to suppress excessive effect of pro-inflammatory cytokine (IL-6).