Association between diarrhea-predominant irritable bowel syndrome and HTR3A, HTR3E gene polymorphism in Yangzhou,Jiangsu province, China

Abstract

To investigate the relationship between diarrhea-predominant irritable bowel syndrome (D-IBS) and HTR3A, HTR3E gene polymorphism in Yangzhou,Jiangsu province. Polymerase chain reaction (PCR) amplification and restriction fragment length polymorphism (RFLP)technique were used to detect the 5'-UTR variant c.-42C > T of HTR3A and 3'-UTR variant c. ∗ 76G > A of HTR3E on 300 healthy subjects and 450 patients with D-IBS. There was significant difference noticed between the D-IBS patients and the controls in the genotype of c.-42C > T sites (P<0.05), while the frequency of T allele was significantly higher in both female and male patients than that in the controls (P < 0.05). The frequencies of G/A genotype and A allele of c. ∗ 76G > A sites were significantly higher in the female-patient group than that in the controls (P < 0.05), however, there was no significant difference between those male patients and controls(P > 0.05). When comparing to the CC genotype of rs1062613 site and GG genotype of rs 62625044 site, the TT and GA genotype were closely related to the D-IBS, with the risk coefficients as 0.29 (95% CI:0.14-0.61)and 0.62 (95% CI:0.39-0.99), with statistically significant differences. T allele and GA genotype might respectively serve as the predisposing factors of D-IBS and on the female D-IBS patients.