Abstract
Background. Squamous cell lung cancer (SCLC) is the most common form of lung cancer among men smokers. Mediators and products of inflammation can contribute to the initiation of carcinogenesis in smokers. The aim of study is to investigate the association between IL1B(rs1143634, rs16944), IL1RN (rs2234663), TNFA (rs1800629), IL6 (rs1800795), IL10 (rs1800896) genes and SCLC depending on the duration of smoking in men.
 Materials and methods. We studied 324 patients with SCLC and 168 healthy men smokers. The typing of the IL1RN(rs2234663) gene polymorphism has analyzed by polymerase chain reaction (PCR). Detection of the IL1B (rs1143634) and IL6 (rs1800795) polymorphisms have performed by restriction fragment length analysis, as a restriction enzyme TaqI was used. The IL1B (rs16944), TNFA(rs1800629) and IL10 (rs1800896) genes polymorphisms have determined through TaqMan-real-time PCR.
 Results. The study showed, the age, duration of smoking and the number of cigarettes smoked per day were the main risk factors for SCLC in men (p 0.0001). The IL6 -174G allele was detected an additional risk factor of SCLC at a smoking duration of less than 35 years (OR = 1.68; 95 % CI: 1.12-2.51; pcor = 0,04). No association of IL1B (rs1143634, rs16944), IL1RN (rs2234663), TNFA (rs1800629), and IL10 (rs1800896) genes with the risk of SCLC in men was identified.
 Conclusion. The duration of smoking and rs1800795 IL6 gene polymorphism may influence on the susceptibility to SCLC in men. Our results can be useful in understanding the molecular mechanisms of development of SCLC.
Highlights
CC Изучали влияние полиморфных локусов interleukin 1 beta (IL1B), IL1RN, TNFA, IL6 и IL10 на риск развития плоскоклеточного рака легкого (ПРЛ) у мужчин в зависимости от длительности курения
Association between cytokine gene polymorphisms and squamous cell lung cancer depending on the duration of smoking in men
1 Federal Research Center of Coal and Coal Chemistry of Siberian Branch of RAS (Institute of Human Ecology SB RAS), Kemerovo, Russia; 2 Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russia; 3 Regional Clinical Hospital, Genetic Consultation, Kemerovo, Russia; 4 Regional Clinical Oncological Hospital, Kemerovo, Russia; 5 Kemerovo Regional Blood Centre, Kemerovo, Russia; 6 Kemerovo State University, Kemerovo, Russia; For citation: Gordeeva LA, Mun SA, Voronina EN, et al Association between cytokine gene polymorphisms and squamous cell lung cancer depending on the duration of smoking in men
Summary
Ассоциации полиморфизма в генах цитокинов с риском плоскоклеточного рака легкого у мужчин в зависимости от длительности курения // Экологическая генетика. CC Изучали влияние полиморфных локусов IL1B (rs1143634, rs16944), IL1RN (rs2234663), TNFA (rs1800629), IL6 (rs1800795) и IL10 (rs1800896) на риск развития плоскоклеточного рака легкого (ПРЛ) у мужчин в зависимости от длительности курения. Целью настоящей работы явилось изучение ассоциации полиморфных локусов rs1143634 и rs16944 в гене IL1B, rs2234663 в гене IL1RN, rs1800629 в гене TNFA, rs1800795 в гене IL6 и rs1800896 в гене IL10 с риском ПРЛ у мужчин в зависимости от длительности курения. РЕЗУЛЬТАТЫ Для анализа ассоциаций с риском ПРЛ были выбраны шесть полиморфных локусов в генах IL1B, IL1RN, TNFA, IL6 и IL10 согласно данным литературы об их функциональной значимости Изучали влияние следующих факторов на риск развития ПРЛ: возраст, длительность и интенсивность курения, полиморфизм в генах цитокинов (табл. 2)
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