Abstract
To evaluate the rs42524 polymorphism of the procollagen type I alpha (α) 2 (COL1A2) gene as a factor related to the development of pelvic organ prolapse (POP) in Brazilian women. The present study involved 112 women with POP stages III and IV (case group) and 180 women with POP stages zero and I (control group). Other clinical data were obtained by interviewing the patients about their medical history, and blood was also collected from the volunteers for the extraction of genomic DNA. The promoter region of the COL1A2 gene containing the rs42524 polymorphism was amplified, and the discrimination between the G and C variants was performed by digestion of the polymerase chain reaction (PCR) products with the MspA1I enzyme followed by agarose gel electrophoresis analysis. A total of 292 women were analyzed. In the case group, 71 had the G/G genotype, 33 had the G/C genotype, and 7 had the C/C genotype. In turn, the ratio in the control group was 117 G/G, 51 G/C, and 11 C/C. There were no significant differences between the groups. Our data did not show an association between the COL1A2 polymorphism and the occurrence of POP.
Highlights
Pelvic organ prolapse (POP) is a pathological condition characterized by the displacement of pelvic viscera in the caudal direction, towards the genital hiatus
The promoter region of the COL1A2 gene containing the rs42524 polymorphism was amplified, and the discrimination between the G and C variants was performed by digestion of the polymerase chain reaction (PCR) products with the MspA1I enzyme followed by agarose gel electrophoresis analysis
Our data did not show an association between the COL1A2 polymorphism and the occurrence of POP
Summary
Pelvic organ prolapse (POP) is a pathological condition characterized by the displacement of pelvic viscera in the caudal direction, towards the genital hiatus. The standardization of its symptoms was updated in 2016 by the International Continence Society (ICS) and by the International Urogynecology Association (IUGA). They considered the following complaints to be related to POP: vaginal bulging, pelvic or suprapubic pressure, bleeding, discharge and infections related to ulceration, need for manual maneuvers to facilitate defecation or urination, and pain in the sacral spine region.[1]. The etiology of POP is considered multifactorial, and several risk factors, such as advanced age, pregnancy, obesity, neuropathies, ethnicity, hysterectomy, instrumental delivery, and menopause, have been identified. The high prevalence of POP in patients with type I and III collagen disorders, such as Ehlers-Danlos and Marfan syndromes, corroborates the importance of studying the genetic background of patients with the disease.[3,4]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Revista Brasileira de Ginecologia e Obstetrícia / RBGO Gynecology and Obstetrics
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
