Association between CETP Taq1B and LIPC -514C/T polymorphisms with the serum lipid levels in a group of Tehran's population: a cross sectional study

Mohammad Ali Kashani Farid,Mehdi Hedayati,Fereidoun Azizi,Fereydoun Siassi,Ahmad Reza Shamshiri,Maryam S Daneshpour

doi:10.1186/1476-511x-9-96

Abstract

BackgroundLow level of high density lipoprotein cholesterol (HDL-C) has high prevalence in the Tehran Lipid and Glucose Study (TLGS) cohort. About 50% of the inter-individual variation in serum HDL-C levels is genetically determined. Polymorphisms in cholesteryl ester transfer protein (CETP) and hepatic lipase (LIPC) genes have been found to be associated with the metabolism and serum concentration of the HDL-C.ObjectivesTo determine the association between Taq1B polymorphism in CETP gene and -514C/T polymorphism in LIPC gene with serum lipid levels and lipid peroxidation in a subgroup of the TLGS population.ResultsSerum HDL-C level had significant association with CETP Taq1B polymorphism and B2B2 subjects had the highest HDL-C levels compared to B2B1 and B1B1 genotypes (37.9 vs. 36.9 and 35.3 mg/dl, respectively; P = 0.01). However, carriers of "B1" allele, in comparison to the non carriers (B2B2), had significantly lower levels of TC (200.1 vs. 215.2 mg/dl; P = 0.005), HDL-C (35.8 vs. 37.9 mg/dl; P = 0.009) and malondialdehyde MDA (4.5 vs. 5.0 nmol/mL; P=0.031). Carriers of the "T" allele in -514C/T polymorphism in LIPC gene had higher means of HDL-C than non carriers (37.7 vs. 35.7 mg/dl, P = 0.04). No other association was found between -514C/T polymorphism and any other serum lipids or MDA level.ConclusionThis study demonstrates the association between Taq1B and -514C/T polymorphisms in the CETP and LIPC genes with the serum HDL-C levels.

Highlights

Coronary artery disease (CAD) and stroke are the leading causes of morbidity, mortality and disability in industrialized countries, and the prevalence of these diseases is increasing rapidly in developing countries[1].Convincing clinical evidence has shown an association between the incidence of CAD and low levels of high density lipoprotein cholesterol (HDL-C) [2,3]
Serum HDL-C level had significant association with cholesteryl ester transfer protein (CETP) Taq1B polymorphism and B2B2 subjects had the highest HDL-C levels compared to B2B1 and B1B1 genotypes (37.9 vs. 36.9 and 35.3 mg/dl, respectively; P = 0.01)
No other association was found between -514C/T polymorphism and any other serum lipids or MDA level

Summary

Introduction

Coronary artery disease (CAD) and stroke are the leading causes of morbidity, mortality and disability in industrialized countries, and the prevalence of these diseases is increasing rapidly in developing countries[1].Convincing clinical evidence has shown an association between the incidence of CAD and low levels of HDL-C [2,3]. Polymorphisms in cholesteryl ester transfer protein (CETP) [9,10] and hepatic lipase (LIPC) [11,12] genes have been found to be associated with the variations in the HDL-C concentration. Cholesteryl Ester Transfer Protein (CETP) is a hydrophobic glycoprotein that circulates in plasma bound mainly to HDL-C[13]. It promotes the redistribution of cholesteryl esters, triglycerides, and, to a lesser extent, phospholipids between plasma lipoproteins[14]. Polymorphisms in cholesteryl ester transfer protein (CETP) and hepatic lipase (LIPC) genes have been found to be associated with the metabolism and serum concentration of the HDL-C

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