Association between BMI and gene expression of anti-Müllerian hormone and androgen receptor in human granulosa cells in women with and without polycystic ovary syndrome.

Abstract

Anti-Müllerian hormone (AMH) is one of the most reliable markers of ovarian reserve. There is evidence which suggests that BMI may be associated with gene expression of AMH, AMH type II receptor (AMHR-II) and androgen receptor (AR) in human granulosa cells (GC) in women with and without polycystic ovary syndrome (PCOS). To investigate the association between BMI and gene expression of AMH, AMHR-II and AR in human GC in women with and without PCOS. In a cross-sectional study, hormonal profiles were measured among 38 patients with PCOS and 38 subjects without PCOS aged 18-40. AMH, AMHR-II and AR mRNA levels were quantified in cumulus GC. Pearson correlation and multiple linear regressions were used to assess the relationships. Quantitative RT-PCR demonstrated that AMH and AMHR-II expression were negatively correlated with BMI (r=-0·39, P<0·001 for AMH and r=-0·49, P<0·001 for AMHR-II), whereas AR expression was positively correlated with BMI (r=0·46, P<0·001). There is a negative association between AMH, AMHR-II expression and BMI, and a positive association between AR expression and BMI in the GC of PCOS and non-PCOS women.