Association between A986S polymorphism of CaSR gene and concentration of 25-OH vitamin D in healthy syrians

Abstract

The calcium Sensing Receptor (CaSR) and vitamin D are considered as essential factors for regulation and sensing the minor changes in calcium concentrations in the extracellular matrix of cells. It is still unclear whether single nucleotide polymorphisms (SNPs) of CaSR gene have effects on vitamin D level. The aim of this study is to evaluate the relationship between CaSR gene polymorphism A986S and 25-OH vitamin D levels in healthy Syrian population. MethodsA total of 197 healthy Syrians individuals (Aged 18 to 50 years. Female: 131 and male: 66) were recruited in this study. Based on vitamin D serum concentration in serum, participants were divided into four groups: sufficiency (G1) (Vit.D > 30 ng/ml), insufficiency (G2) (Vit.D: 20–30 ng/ml), deficiency (G3) (Vit.D: 10–20 ng/ml), and severe deficiency (G4) (Vit.D < 10 ng/ml). All the subjects were genotyped at the CaSR A986S with PCR followed by SacI restriction enzyme digestion and agarose gel electrophoresis. ResultsThe frequency of genotypes A986S for the whole study participants was 59.9% (AA), and 35.5% (AS) and 4.6% (SS). Allele frequencies for A and S were 77.7% and 22.3% respectively. Frequencies of AA, AS and SS genotypes were 94.1%, 5.9% and 0% in sufficient vitamin D group, 71.1%, 26.1% and 2.2% in vitamin D insufficiency group, 60.4%, 33.3% and 6.3% in vitamin D deficiency group, and 34.8%, 58% and 7.2% in severe deficiency of vitamin D respectively. The differences between G1 group and rest groups G2, G3, G4 were statistically significant and P value were (0.038, 0.002, <0.001), respectively. In summary, there was a strong correlation between allele S and decreased vitamin D levels. Additionally, the study reported an association between normal vitamin D levels and AA genotype of the CaSR gene in healthy Syrians.